Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-00371

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL
View all Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Precision

Intra-Assay Precision (Precision within an assay) 5.61%

Inter-Assay Precision (Precision between assays) 4.92%

Recovery

Recovery

87 - 106%

Linearity

Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric)

Scientific Data Images

Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric)

ELISA: Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric) [NBP3-00371] -

ELISA: Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric) [NBP3-00371] - Standard Curve Reference

Kit Contents

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Alternate Names

CTSA, Lysosomal Carboxypeptidase A

Gene Symbol

CTSA

Additional Cathepsin A/Lysosomal Carboxypeptidase A Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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