CBS Antibody (519) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43604

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Bovine (94%), Rabbit (95%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 519

Format

Azide and BSA Free
View all CBS Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (93%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CBS Antibody (519) - Azide and BSA Free

Knockout Validated: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604] - Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blot: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (519) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604]

Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604]

Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604] - Paraffin-embedded mouse kidney. CBS antibody [519] diluted at 1:250.
Western Blot: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604] - Analysis of A. 30 ug Raji whole cell lysate/extract B. 30 ug K562 whole cell lysate/extract 7.5 % SDS-PAGE CBS antibody [519] dilution: 1:1000
Western Blot: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604] - Analysis of A. 50 ug mouse liver lysate/extract 7.5 % SDS-PAGE CBS antibody [519] dilution: 1:2000.
Western Blot: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604]

Western Blot: CBS Antibody (519) [NBP2-43604] - Analysis of A. 50 ug rat liver lysate/extract 7.5 % SDS-PAGE CBS antibody [519] dilution: 1:1000
Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604]

Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604]

Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604] - Paraffin-embedded rat liver. CBS antibody [519] diluted at 1:250.
CBS Antibody (519) - Azide and BSA Free

Western Blot: CBS Antibody (519) [NBP2-43604] -

Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (NBP2-43604) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
CBS Antibody (519) - Azide and BSA Free

Western Blot: CBS Antibody (519) [NBP2-43604] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (NBP2-43604) diluted at 1:500.

Applications for CBS Antibody (519) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CBS

CBS is encoded by this gene is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.

Alternate Names

cystathionine-beta-synthase, EC 4.2.1.2210Beta-thionase

Gene Symbol

CBS

Additional CBS Products

Product Documents for CBS Antibody (519) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CBS Antibody (519) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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