CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44561

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Human

Format

Azide and BSA Free
View all CD123/CD3 (Flotetuzumab) Primary Antibodies »

Product Specifications

Immunogen

CD123/CD3 (Uniprot: P26951 & P07766)

Clonality

Monoclonal

Host

Human

Theoretical MW

117.82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free

CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free

Flow Cytometry: CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free [NBP3-44561]

Flotetuzumab bound to huCD3e-jurkat cells, and then rebounded to fluorescent secondary antibodies (PE Streptavidin ), and test by flow cytometry. As shown in fig, Flotetuzumab bound to huCD3e-jurkat cells, and the EC50 was 33.800 nM.
CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free

Functional: CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free [NBP3-44561]

Co-incubation of Flotetuzumab with Jurkat cells, then with the addition of GSV0-hu-CD123-ECD-Fc cells for 6 hours. Bright-Lite was used to detect the fluorescent signal.. As shown in fig, Flotetuzumab was able to activate the NF-AT signaling pathway, and the EC50 was 4.555 nM.
CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free

Flow Cytometry: CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free [NBP3-44561]

Flotetuzumab-Biotin bound to GSV0-hu-CD123-ECD-Fc cells, and then rebounded to fluorescent secondary antibodies (PE Streptavidin ), and test by flow cytometry. As shown in fig, FlotetFlotetuzumab-Biotin bound to GSV0-hu-CD123-ECD-Fc cells, and the EC50 was 31.24 nM.

Applications for CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

> 90% by SDS-PAGE and HPLC

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD123/CD3 (Flotetuzumab) Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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