Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunoprecipitation, Cell depletion, T Cell Stimulation, CyTOF-reported

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Recombinant Monoclonal Hamster IgG Clone # 145-2C11
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Product Specifications

Immunogen

H-2Kb-specific mouse cytotoxic T-lymphocyte

Specificity

Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.1 Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.3,4

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Scientific Data Images for CD3 epsilon Antibody (145-2C11) [FITC]

CD3 epsilon Antibody (145-2C11) [FITC]

CD3 epsilon Antibody (145-2C11) [FITC] [FAB484F] -

CD3 epsilon Antibody (145-2C11) [FITC] [FAB484F] - Vial of FITC conjugated antibody. FITC is optimally excited at 498 nm by the Blue laser (488 nm) and has an emission maximum of 519 nm.

Applications for CD3 epsilon Antibody (145-2C11) [FITC]

Application
Recommended Usage

Cell depletion

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-reported

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

T Cell Stimulation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (145-2C11) [FITC]

Certificate of Analysis

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Product Specific Notices for CD3 epsilon Antibody (145-2C11) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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