Mouse CD3 epsilon Antibody

(10 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.1 Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.3,4
  • Source
    Monoclonal Hamster IgG Clone # 145-2C11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    H-2Kb-specific mouse cytotoxic T-lymphocyte
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Mouse splenocytes
  • Immunoprecipitation
    Leo, O. et al. (1987) Proc. Natl. Acad. Sci. 84:1374.
  • Cell depletion
    Small, M. et al. (1994) J. Immunol. Meth. 167:103.
  • CyTOF-reported
    Fienberg, H.G. et al. (2012) Cytometry 81: 467. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • T Cell Stimulation
    0.1-0.6 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
T Cell Stimulation
Mouse CD3 epsilon  Antibody Stimulates Cell Proliferation in Mouse T Cells. Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) stimulates cell proliferation in mouse T cells in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 0.1‑0.6 μg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3 epsilon
CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.4 The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.1 145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.6, 7
  • References:
    1. Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374.
    2. Portoles, P. et al. (1989) J. Immunol. 142:4169.
    3. Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703.
    4. Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583.
    5. Small, M. et al. (1994) J. Immunol. Meth. 167:103.
    6. Alegre, M.L. et al. (1991) J. Immunol. 146:1184.
    7. Hendrickson, M. et al. (1995) Transplantation 60:828.
  • Entrez Gene IDs:
    916 (Human); 12503 (Mouse)
  • Alternate Names:
    CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors
    Authors: H Kinosada, JI Yasunaga, K Shimura, P Miyazato, C Onishi, T Iyoda, K Inaba, M Matsuoka
    PLoS Pathog, 2017;13(1):e1006120.
  2. Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis
    Authors: LT McDonald, SD Johnson, DL Russell, MRI Young, AC LaRue
    PLoS ONE, 2017;12(7):e0180724.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Developmental heterogeneity in DNA packaging patterns influences T-cell activation and transmigration.
    Authors: Gupta S, Marcel N, Talwar S, Garg M, R I, Perumalsamy L, Sarin A, Shivashankar G
    PLoS ONE, 2012;7(9):e43718.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  4. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
    Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
    FASEB J., 2011;25(1):409-19.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
    Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
    J. Immunol., 2010;184(4):2130-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  6. Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking.
    Authors: Sinclair LV, Finlay D, Feijoo C, Cornish GH, Gray A, Ager A, Okkenhaug K, Hagenbeek TJ, Spits H, Cantrell DA
    Nat. Immunol., 2008;9(5):513-21.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  7. Protection of CD8+ T cells from activation-induced cell death by IL-18.
    Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
    J. Leukoc. Biol., 2007;82(1):142-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  8. Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells.
    Authors: Bheeshmachar G, Purushotaman D, Sade H, Gunasekharan V, Rangarajan A, Sarin A
    J. Immunol., 2006;177(8):5041-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  9. Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay.
    Authors: Ogilvie RL, Abelson M, Hau HH, Vlasova I, Blackshear PJ, Bohjanen PR
    J. Immunol., 2005;174(2):953-61.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  10. Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
    Authors: Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M
    J. Neurosci., 2004;24(27):6133-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
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