Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunoprecipitation, Cell depletion, T Cell Stimulation, CyTOF-reported

Cited:

Neutralization, Bioassay, Functional Assay, Immunoassay Development, MACS

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # 145-2C11
View all CD3 epsilon Primary Antibodies »

Product Specifications

Immunogen

H-2Kb-specific mouse cytotoxic T-lymphocyte

Specificity

Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.1 Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.3,4

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CD3 epsilon Antibody

Detection of CD3 epsilon in mouse splenocytes by Flow Cytometry

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) or (B) Hamster IgG control antibody followed by Allophycocyanin-conjugated Anti-Hamster IgG Secondary Antibody (Catalog # F0121). View our protocol for Staining Membrane-associated Proteins.

Mouse CD3e Antibody Stimulates Cell Proliferation antibody in Mouse T Cells.

Mouse CD3 epsilon Antibody Stimulates Cell Proliferation in Mouse T Cells.

Hamster Anti-Mouse CD3e Monoclonal Antibody (Catalog # MAB484) stimulates cell proliferation in mouse T cells in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 0.1-0.6 µg/mL.

Detection of CD3 epsilon by Flow Cytometry

Detection of CD3 epsilon by Flow Cytometry

Flt3L induced changes in splenic and nodal lymphocyte populationsduring mBSA arthritis.A) Frequency of CD3+, CD19+ and TFH (within the CD4+ population) cells in the spleen and draining lymph nodes at day 28. B) Proliferative response of isolated splenocytes following in vitro stimulation with mBSA (25 μg/ml), LPS (10 μg/ml) or anti-CD3 (1 μg/ml). Results are presented as fold increase compared to non-stimulated cells. Data are presented as mean ± SEM and statistical significance was assessed using unpaired t-test. *P<.05, **P<.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23349985), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CD3 epsilon Antibody

Application
Recommended Usage

Cell depletion

Small, M. et al. (1994) J. Immunol. Meth. 167:103.

CyTOF-reported

Fienberg, H.G. et al. (2012) Cytometry 81: 467. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: B220+ Mouse splenocytes

Immunoprecipitation

Leo, O. et al. (1987) Proc. Natl. Acad. Sci. 84:1374.

T Cell Stimulation

0.1-0.6 µg/mL
Sample: Mouse splenic T cells

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD3 epsilon

CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.4 The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.1 145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.6, 7

References

  1. Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374.
  2. Portoles, P. et al. (1989) J. Immunol. 142:4169.
  3. Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703.
  4. Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583.
  5. Small, M. et al. (1994) J. Immunol. Meth. 167:103.
  6. Alegre, M.L. et al. (1991) J. Immunol. 146:1184.
  7. Hendrickson, M. et al. (1995) Transplantation 60:828.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for Mouse CD3 epsilon Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD3 epsilon Antibody

For research use only

Citations for Mouse CD3 epsilon Antibody

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