CD3 zeta Antibody (21H6) - BSA Free

Novus Biologicals | Catalog # NBP3-26684

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 21H6 expressed in HEK293

Format

BSA Free
View all CD3 zeta/CD247 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human CD3 zeta [UniProt P20963]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD3 zeta Antibody (21H6) - BSA Free

CD3 zeta Antibody (21H6)

Flow Cytometry: CD3 zeta Antibody (21H6) [NBP3-26684] -

Flow Cytometry: CD3 zeta Antibody (21H6) [NBP3-26684] - Overlay Peak curve showing Jurkat cells stained with CD3 zeta Antibody (21H6) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for CD3 zeta Antibody (21H6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CD3 zeta/CD247

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta Antibody (21H6) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD3 zeta Antibody (21H6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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