CD3 zeta [p Tyr142] Antibody (EM-54) - BSA Free

Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5

Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) - BSA Free [NBP2-37708] -

The anti-CD3 monoclonal antibody does not induce activation in total RAW cells. (A) Immunoblot evaluation of proteins involved in the CD3 signaling pathway; NFAT, phosphorylated C-Jun (pC-Jun), IKK-alpha, NF-kappa B and phosphorylated CD3-zeta chain (pCD3-zeta ), beta -Tubulin as loading control in lysates of RAW cells cultured for 24 h under the following conditions: RPMI (control), anti-CD3 (stimulus), and isotype control. Relative units (R.U) of NFAT, pC-Jun, IKK-alpha, NF-kappa B, and pCD3-zeta in RAW cell lysates ((B–F), respectively). Levels of TNF and IL-6 were measured in supernatants collected from total RAW cells by ELISA sandwich assay ((G,H), respectively). n = 5 independent experiments (immunoblot) and n = 5 independent experiments performed in triplicate (ELISA). Bars represent the mean value +/- SD (B–F) or the mean of means +/- SEM (G,H). Kruskal–Wallis and corrected for the Dunn test were used for immunoblot data and one-way ANOVA followed by Bonferroni test for ELISA. * p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35626672), licensed under a CC-BY license. Not internally tested by Novus Biologicals.