CD300c Antibody (03) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-06451
![Flow Cytometry: CD300c Antibody (03) [NBP3-06451]](https://resources.rndsystems.com/images/products/CD300c-Antibody-03-Flow-Cytometry-NBP3-06451-img0001.jpg "Flow Cytometry: CD300c Antibody (03) [NBP3-06451]")
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Description
Scientific Data Images for CD300c Antibody (03) - Azide and BSA Free
Flow Cytometry: CD300c Antibody (03) [NBP3-06451]
Flow Cytometry: CD300c Antibody (03) [NBP3-06451] - Flow cytometric analysis of Human CD300c expression on human whole blood monocytes. Cells were stained with purified anti-Human CD300c, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.Applications for CD300c Antibody (03) - Azide and BSA Free
Flow Cytometry
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- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
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Background: CD300c
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Additional CD300c Products
Product Documents for CD300c Antibody (03) - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for CD300c Antibody (03) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CD300c Antibody (03) - Azide and BSA Free
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Q: Why are there different molecular weight bands in western blot images for two of your antibodies (NBP1-84432 and H00010871-B01P) and overexpression lysate NBL1-08931?
A: A variety of band patterns can be expected when detecting CD300C. This protein has multiple glycosliation post-translational modifications that will change its molecular weight, and which vary in different experimental conditions. If you look at the uniprot link listed below, you can view more information on the specific PTMs for CD300C:
https://www.uniprot.org/uniprot/Q08708#sequences
We can be assured that these varying band patterns are indeed detection of CD300C by the absence of bands in the negative control. Any non-specific binding would show in both the negative and positive control, which is not the case here.