CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free

Novus Biologicals | Catalog # NBP2-62230

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # UP-H2

Format

Low Endotoxin, Azide and BSA Free
View all CD300e/LMIR6 Primary Antibodies »

Product Specifications

Immunogen

CD300e-HA-transfected cells

Specificity

The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein

Scientific Data Images for CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free

Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free [NBP2-62230]

Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free [NBP2-62230]

Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin [NBP2-62230] - Surface staining of human peripheral blood with anti-human CD300e (UP-H2) PE.
Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free [NBP2-62230]

Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free [NBP2-62230]

Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin [NBP2-62230] - Surface staining of human peripheral blood with anti-human CD300e (UP-H2) APC.
CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free Flow Cytometry: CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free [NBP2-62230]

Flow Cytometry: CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free [NBP2-62230]

Separation of human monocytes (red-filled) from CD300e negative lymphocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood using anti-human CD300e (UP-H2) purified antibody (concentration in sample 4 ug/ml, GAM APC).

Applications for CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD300e/LMIR6

CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.

Alternate Names

CD300e, CD300LE, CLM2, IREM-2, LMIR6

Gene Symbol

CD300E

Additional CD300e/LMIR6 Products

Product Documents for CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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