CD40 Ligand/TNFSF5 Antibody (TRAP1.3.6) - BSA Free

Novus Biologicals | Catalog # NBP1-42527

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TRAP1.3.6

Format

BSA Free
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Product Specifications

Immunogen

Mouse myeloma cell line transfected with human CD40 Ligand/TNFSF5

Epitope

Binds to CD40 Ligand/TNFSF5 at an epitope distinct from the CD40 binding site

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CD40 Ligand/TNFSF5 Antibody (TRAP1.3.6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
The antibody can be used for flow cytometry. Flow Cytometry: Use 10ul of the suggested working dilution (1/10-1/50) to label 106 cells in 100ul. This antibody is CyTOF ready.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD40 Ligand/TNFSF5

CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for CD40 Ligand/TNFSF5 Antibody (TRAP1.3.6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD40 Ligand/TNFSF5 Antibody (TRAP1.3.6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CD40 Ligand/TNFSF5 Antibody (TRAP1.3.6) - BSA Free

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  • Q: I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?

    A:

    Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

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