Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-91184

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (20 uL)

Sensitivity

0.06 ng/mL

Assay Range

0.16 - 10.0 ng/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

The assay detects both natural and recombinant human CD40 Ligand/TNFSF5. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD40 Ligand/TNFSF5 positive sample. There was no cross reactivity or interference.

Description

The human sCD40L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD40L

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.00%

Inter-Assay Precision (Precision between assays) CV% 6.80%

Recovery for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Recovery

91%

Scientific Data Images for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

ELISA: Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) [NBP1-91184]

ELISA: Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) [NBP1-91184]

ELISA: CD40 Ligand/TNFSF5 ELISA Kit [NBP1-91184] - Standard curve

Kit Contents for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD40L
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Blue-Dye
  • Control high, lyophilized
  • Control low, lyophilized
  • Dilution Plates
  • Green-Dye
  • HRP-Conjugate anti-human sCD40L monoclonal antibody
  • Human sCD40L Standard lyophilized, 20 ng/ml upon reconstitution
  • Sample Diluent
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD40 Ligand/TNFSF5

CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.) A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40. CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome is targeted to the CD40 L gene, it is functional involved in B cell hybridomas and chronic lymphocytic leukemia as well as several autoimmune diseases.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

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    • Q: I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
      A: Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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