CD46 Antibody (MEM-258) - BSA Free

Novus Biologicals | Catalog # NB500-301

Novus Biologicals

Key Product Details

Species Reactivity

Human, Bovine

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-258

Format

BSA Free
View all CD46 Primary Antibodies »

Product Specifications

Immunogen

HPB-ALL human T cell line

Specificity

The antibody MEM-258 recognizes an epitope on SCR4 (the membrane-proximal SCR) domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast; it is negative on erythrocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CD46 Antibody (MEM-258) - BSA Free (NB500-301) is a monoclonal antibody validated for use in WB, Flow and IP. Anti-CD46 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD46 Antibody (MEM-258) - BSA Free

Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Staining of human peripheral blood cells with anti-CD46 (MEM-258) Alexa Fluor® 700.
Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Surface staining of human peripheral blood cells with anti-CD46 (MEM-258) PE
Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Surface staining of human peripheral blood cells with anti-CD46 (MEM-258) biotin
Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Staining of human peripheral blood cells with anti-CD46 (MEM-258) Pacific BlueTM.
Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Staining of human peripheral blood cells with anti-CD46 (MEM-258) APC.
Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) - BSA Free [NB500-301]

Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Staining of human peripheral blood cells with anti-CD46 (MEM-258) FITC.

Applications for CD46 Antibody (MEM-258) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Western Blotting - Non-reducing conditions. This antibody is CyTOF ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD46

CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.

Alternate Names

CD46, MCP

Gene Symbol

CD46

Additional CD46 Products

Product Documents for CD46 Antibody (MEM-258) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD46 Antibody (MEM-258) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD46 Antibody (MEM-258) - BSA Free

Customer Reviews for CD46 Antibody (MEM-258) - BSA Free

There are currently no reviews for this product. Be the first to review CD46 Antibody (MEM-258) - BSA Free and earn rewards!

Have you used CD46 Antibody (MEM-258) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies