CD47 Antibody (OX-101) - BSA Free

Novus Biologicals | Catalog # NB100-66632

Novus Biologicals
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Key Product Details

Species Reactivity

Rat

Applications

Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-101

Format

BSA Free
View all CD47 Primary Antibodies »

Product Specifications

Immunogen

Rat thymocytes

Specificity

Antibody recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells. Clone OX-101 has been reported to block the interaction of CD47 and SIRP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD47 Antibody (OX-101) - BSA Free

Flow Cytometry: CD47 Antibody (OX-101) - BSA Free [NB100-66632]

Flow Cytometry: CD47 Antibody (OX-101) - BSA Free [NB100-66632]

Flow Cytometry: CD47 Antibody (OX-101) [NB100-66632] - Staining of Rat Spleen cells with Mouse anti Rat CD47:FITC
Flow Cytometry: CD47 Antibody (OX-101) - BSA Free [NB100-66632]

Flow Cytometry: CD47 Antibody (OX-101) - BSA Free [NB100-66632]

Flow Cytometry: CD47 Antibody (OX-101) [NB100-66632] - Staining of Rat Spleen cells with Mouse anti Rat CD47:RPE
Flow Cytometry: CD47 Antibody (OX-101) - BSA Free [NB100-66632]

Flow Cytometry: CD47 Antibody (OX-101) - BSA Free [NB100-66632]

Flow Cytometry: CD47 Antibody (OX-101) [NB100-66632] - Staining of Rat Spleen cells with Mouse anti Rat CD47:Alexa Fluor® 647

Applications for CD47 Antibody (OX-101) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells. OX-101 (this clone) recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP).

Alternate Names

CD47, IAP, MER6, OA3

Entrez Gene IDs

29364 (Rat)

Gene Symbol

CD47

UniProt

P97829 (Rat)

Additional CD47 Products

Product Documents for CD47 Antibody (OX-101) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD47 Antibody (OX-101) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail