Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 004 expressed in HEK293

Format

Azide and BSA Free
View all CD53 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD53 (Uniprot#: Q61451; Glu107-Asn181).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD53 Antibody (004) - Azide and BSA Free

Western Blot: CD53 Antibody (004) [NBP2-90697]

Western Blot: CD53 Antibody (004) [NBP2-90697]

Western Blot: CD53 Antibody (004) [NBP2-90697] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate2 uL anti-Mouse CD53 Antibody (004) and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-Mouse Mouse CD53 Antibody (004),at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 24 kDa Observed band size: 24 kDa
Western Blot: CD53 Antibody (004) [NBP2-90697]

Western Blot: CD53 Antibody (004) [NBP2-90697]

Western Blot: CD53 Antibody (004) [NBP2-90697] - Anti-CD53 rabbit monoclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:15 kDa

Applications for CD53 Antibody (004) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

0.5-2 ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD53

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Alternate Names

CD53, MOX44, TSPAN25

Gene Symbol

CD53

Additional CD53 Products

Product Documents for CD53 Antibody (004) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD53 Antibody (004) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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