CD55/DAF Antibody (BRIC216) - BSA Free

Novus Biologicals | Catalog # NB100-65914

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BRIC216

Format

BSA Free
View all CD55/DAF Primary Antibodies »

Product Specifications

Immunogen

Human fibroblast cell line

Specificity

NB100-65914 recognizes the CD55 antigen. BRIC 216 recognizes the consensus region 3 of the DAF molecule, which contains the functional site, and the antibody blocks the function of DAF.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CD55/DAF Antibody (BRIC216) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
The antigen is pronase and trypsin resistant and chymotrypsin sensitive. This antibody has a functional binding affinity to erythrocytes of 8.7 x 10^7 M^-1. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

TRIS buffered saline, 100mM glycine

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD55/DAF

Avoidance by host tissues of attack by autologous complement proteins is dependent in part on the activities of membrane regulatory factors. One molecule involved in this control is a 70 kD glycoprotein termed decay accelerating factor (DAF), or CD55. Interruption by CD55 of the complement sequence at an early step in activation effectively halts progression of the cascade and prevents consequent cell injury. In man, CD55 is expressed on the plasma membrane of all cell types that are in intimate contact with plasma complement proteins. This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. CD55 also acts as the receptor for echovirus 7 and related viruses(echoviruses 13, 21, 29 and 33). It is attached to the membrane by a GPI-anchor.

Alternate Names

CD55, CR, DAF, TC

Entrez Gene IDs

1604 (Human)

Gene Symbol

CD55

UniProt

P08174

Additional CD55/DAF Products

Product Documents for CD55/DAF Antibody (BRIC216) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD55/DAF Antibody (BRIC216) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Complement Pathway

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Protocols

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