CD59 Antibody (28D4) - BSA Free

Novus Biologicals | Catalog # NBP3-26676

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 28D4 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from Human CD59 [UniProt P13987]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD59 Antibody (28D4) - BSA Free

CD59 Antibody (28D4)

Flow Cytometry: CD59 Antibody (28D4) [NBP3-26676] -

Flow Cytometry: CD59 Antibody (28D4) [NBP3-26676] - Overlay Peak curve showing Hela cells stained with CD59 Antibody (28D4) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for CD59 Antibody (28D4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CD59

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Alternate Names

CD59, HRF20, MACIF, MIC11, Protectin

Gene Symbol

CD59

Additional CD59 Products

Product Documents for CD59 Antibody (28D4) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD59 Antibody (28D4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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