CD69 Antibody (30H2) - BSA Free

Novus Biologicals | Catalog # NBP3-26675

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG4 (S228P) Clone # 30H2 expressed in HEK293

Format

BSA Free
View all CD69 Primary Antibodies »

Product Specifications

Immunogen

Recombinant Human CD69 protein [UniProt Q07108]

Clonality

Monoclonal

Host

Human

Isotype

IgG4 (S228P)

Scientific Data Images for CD69 Antibody (30H2) - BSA Free

CD69 Antibody (30H2)

ELISA: CD69 Antibody (30H2) [NBP3-26675] -

ELISA: CD69 Antibody (30H2) [NBP3-26675] - The Binding Activity of Human CD69 with CD69 Antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD69 at 2 ug/mL can bind CD69 Antibody, the EC50 is 23.17-26.04 ng/mL.
CD69 Antibody (30H2)

Flow Cytometry: Human CD69 mAb (30H2) [NBP3-26675]

Flow Cytometry: Human CD69 mAb (30H2) [NBP3-26675] - Untransfected CHO-K1 cells (green line) and transfected Human CD69 CHO-K1 stable cells (red line) were stained with anti-CD69 antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Applications for CD69 Antibody (30H2) - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:50000

Flow Cytometry

1:50-1:200

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% Glycerol, 0.01M PBS, PH 7.4

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CD69

CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. (1,2) It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. (3) CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. (1-4) Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils. (4) Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. (4,5) MAb H1.2F3 augments PMA-induced T-cell proliferation,1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells. (7)

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Gene Symbol

CD69

Additional CD69 Products

Product Documents for CD69 Antibody (30H2) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD69 Antibody (30H2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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