CD83 Antibody (DMC436) - Chimeric

Novus Biologicals | Catalog # NBP3-48334

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC436
View all CD83 Primary Antibodies »

Product Specifications

Immunogen

CD83 recombinant protein expressed in mammalian cells (Accession# Q01151). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for CD83 Antibody (DMC436) - Chimeric

CD83 Antibody (DMC436) - Chimeric

Flow Cytometry: CD83 Antibody (DMC436) - Chimeric [NBP3-48334]

Flow cytometry analysis of antigen binding of anti-human CD83 mAb(NBP3-48334). (A) NBP3-48334 does not bind to CHO-S cells that do not express CD83. (B) A clear peak shift of NBP3-48334 was seen compared to the control when incubated with CD83-expressing Raji cells, indicating strong binding of NBP3-48334 to CD83. Antibodies were incubated at 5 ug/mL.
CD83 Antibody (DMC436) - Chimeric

Flow Cytometry: CD83 Antibody (DMC436) - Chimeric [NBP3-48334]

Flow cytometry analysis with Anti-CD83 (DMC436) on HEK293 cells transfected with human CD83 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Applications for CD83 Antibody (DMC436) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD83

CD83 is a 43 kD single chain type I glycoprotein also known as HB15. A member of the immunoglobulin superfamily, CD83 is expressed on a subset of dendritic cells, Langerhans cells, and weakly on activated lymphocytes. Although CD83 is thought to play a role in antigen presentation and/or lymphocyte activation, the precise function of this protein is unknown. CD83 is considered to be a useful marker for mature dendritic cells.

Alternate Names

BL11, CD83, HB15

Gene Symbol

CD83

Additional CD83 Products

Product Documents for CD83 Antibody (DMC436) - Chimeric

Certificate of Analysis

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Product Specific Notices for CD83 Antibody (DMC436) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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