CD9 Antibody (IVA50) - BSA Free

Novus Biologicals | Catalog # NB500-494

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Bovine

Cited:

Human, Bovine

Applications

Validated:

Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # IVA50

Format

BSA Free
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Product Specifications

Immunogen

Bovine thrombocytes.

Specificity

The antibody IVA50 reacts with CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD9 Antibody (IVA50) - BSA Free

Immunoprecipitation: CD9 Antibody (IVA50) - BSA Free [NB500-494]

Immunoprecipitation: CD9 Antibody (IVA50) - BSA Free [NB500-494]

Immunoprecipitation: CD9 Antibody (IVA50) [NB500-494] - Fig. 1. Immunoprecipitation of human CD9 from the biotin-labeled human platelets lysates. Lane 1: immunoprecipitation with anti-CD9 (IVA50) Lane 2: immunoprecipitation with anti-human CD9 control antibody Lane 3: immunoprecipitation with negative control.
CD9 Antibody (IVA50) - BSA Free Flow Cytometry: CD9 Antibody (IVA50) - BSA Free [NB500-494]

Flow Cytometry: CD9 Antibody (IVA50) - BSA Free [NB500-494]

Separation of bovine CD9 positive thrombocytes (red-filled) from lymphocytes (black-dashed) in Analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) purified antibody (concentration in sample 10 ug/ml) GAM APC.
CD9 Antibody (IVA50) - BSA Free

Western Blot: CD9 Antibody (IVA50) - BSA Free [NB500-494] -

(a) Mean fluorescence (log scaled) of mEVs under confocal microscopy loaded with Calcein-AM post-lyophilization after removal of exogenous dye via Sepharose G50 spin column. N = 6 images, error bars are representative of standard deviation within each group; biological duplicates. No signal was observed in HEPES control, 10mM TH, and 50mM sucrose groups (Images in Figure S6). Multiple T tests with Bonferroni Correction ( alpha = 0.05), reveals significant differences between fresh and 25mM TH | 75mM TH and between 25 mM TH and 50mM TH | 50mM TH + W. N = 6 images, 3 ROIs each (b) CTDR: DAPI fluorescent ratios (log-scaled) of CTDR loaded fresh mEVs supplemented with TH (50mM) and TH (50mM) + Tryptophan (W; 100 uM) on MDCK cells and 50mM TH + 100 μm Tryptophan mEVs loaded with CTDR uptake post-lyophilization over time in room temperature storage, biological triplicates with N = 10 pictures per sample, outliers removed. (d) Western blotting of lyophilized mEV isolate for CD9, TSG-101, and negative expression of Calnexin. Lyophilized samples were stored at RT for 1 month Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39806456), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CD9 Antibody (IVA50) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:50

Western Blot

1 ug/ml
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD9

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Gene Symbol

CD9

Additional CD9 Products

Product Documents for CD9 Antibody (IVA50) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD9 Antibody (IVA50) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD9 Antibody (IVA50) - BSA Free

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Protocols

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FAQs

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