CD99 Antibody (23GB3395)

Novus Biologicals | Catalog # NBP3-51096

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3395
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Product Specifications

Immunogen

A synthesized peptide derived from human CD99 (Uniprot: P14209)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD99 Antibody (23GB3395)

CD99 Antibody (23GB3395)

Flow Cytometry: CD99 Antibody (23GB3395)[NBP3-51096] -

Validation of CD99 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CD99 antibody (1:2,000) and analyzed using BD flow cytometer.
CD99 Antibody (23GB3395)

Flow Cytometry: CD99 Antibody (23GB3395)[NBP3-51096] -

Flow cytometric analysis of CD99expression in HepG2 cells using CD99 antibody (1:2,000). Green, isotype control; red, CD99.
CD99 Antibody (23GB3395)

Western Blot: CD99 Antibody (23GB3395)[NBP3-51096] -

Western blotting analysis using CD99 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
CD99 Antibody (23GB3395)

Western Blot: CD99 Antibody (23GB3395)[NBP3-51096] -

Western blotting analysis using CD99 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for CD99 Antibody (23GB3395)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD99

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

Additional CD99 Products

Product Documents for CD99 Antibody (23GB3395)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD99 Antibody (23GB3395)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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