CDC2/CDK1 [p Thr161] Antibody

Novus Biologicals | Catalog # NB100-81837

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CDC2/CDK1 Primary Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human CDC2/CDK1 around the phosphorylation site of threonine 161 (T-Y-TP-H-E).

Modification

p Thr161

Specificity

Detects endogenous levels of CDC2/CDK1 only when phosphorylated at threonine 161.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDC2/CDK1 [p Thr161] Antibody

Western Blot: CDC2/CDK1 [p Thr161] Antibody [NB100-81837]

Western Blot: CDC2/CDK1 [p Thr161] Antibody [NB100-81837]

Western Blot: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] - Analysis of extracts from Hela cells untreated (lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Thr161) antibody.

Immunohistochemistry-Paraffin: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] -

Immunohistochemistry-Paraffin: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81837 (left) or the same antibody preincubated with blocking peptide(right).
CDC2/CDK1 [p Thr161] Antibody

Immunohistochemistry-Paraffin: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] -

Immunohistochemistry-Paraffin: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81837(left) or the same antibody preincubated with blocking peptide(right).

Applications for CDC2/CDK1 [p Thr161] Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC2/CDK1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Entrez Gene IDs

983 (Human); 12534 (Mouse); 54237 (Rat)

Gene Symbol

CDK1

UniProt

P06493 (Human)

Additional CDC2/CDK1 Products

Product Documents for CDC2/CDK1 [p Thr161] Antibody

Certificate of Analysis

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Product Specific Notices for CDC2/CDK1 [p Thr161] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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Protocols

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