Cdc20 Antibody

Novus Biologicals | Catalog # NB100-59828

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Cdc20 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 449 and 499 of human cell division cycle 20 using the numbering given in entry NP_001246.2 (GeneID 991).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cdc20 Antibody

Western Blot: Cdc20 Antibody [NB100-59828]

Western Blot: Cdc20 Antibody [NB100-59828]

Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Western Blot: Cdc20 Antibody [NB100-59828]

Western Blot: Cdc20 Antibody [NB100-59828]

Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human CDC20 on HeLa whole cell lysate using NB100-59828. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody NB100-59827.
Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB.
Immunoprecipitation: Cdc20 Antibody [NB100-59828]

Immunoprecipitation: Cdc20 Antibody [NB100-59828]

Immunoprecipitation: Cdc20 Antibody [NB100-59828] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59828 (lot NB100-59828-2) used for IP at 6 ug per reaction. CDC20 was also immunoprecipitated by a previous lot of this antibody (lot NB100-59828-1) and rabbit anti-CDC20 antibody NB100-59827. For blotting immunoprecipitated CDC20, NB100-59828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Cdc20 Antibody

Western Blot: Cdc20 Antibody [NB100-59828] -

Effects of YBX1 knockdown by siRNA#1 and siRNA#3 on protein expression of cell cycle-related genes. (A) Expression of cell cycle-related genes as assessed by western blot analysis. (B) Effects of YBX1 siRNAs (siRNA#1 and siRNA#3) on the expression of cell cycle-related genes in cell lines following transfection with YBX1 siRNAs for 3 days. alpha -tubulin was used as a loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39289424), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Cdc20 Antibody

Western Blot: Cdc20 Antibody [NB100-59828] -

Effects of YBX1 knockdown by siRNA#1 and siRNA#3 on protein expression of cell cycle-related genes. (A) Expression of cell cycle-related genes as assessed by western blot analysis. (B) Effects of YBX1 siRNAs (siRNA#1 and siRNA#3) on the expression of cell cycle-related genes in cell lines following transfection with YBX1 siRNAs for 3 days. alpha -tubulin was used as a loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39289424), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Cdc20 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cdc20

CDC20 is a WD repeat containing cell cycle-regulator that binds to and activates the ubiquitin-ligase activity of the APC/c (anaphase-promoting complex/cyclosome). APC/c ubiquitinating activity is responsible for the degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. CDC20 has also been reported to form a complex with histone deacetylases and can function as a transcriptional repressor involved in cell cycle arrest. As a regulator of APC/c activity, CDC20 plays a key role in the spindle-assembly checkpoint.

Long Name

Cell division cycle protein 20 homolog

Alternate Names

p55CDC

Entrez Gene IDs

991 (Human)

Gene Symbol

CDC20

UniProt

NP_001246.2 (Human)

Additional Cdc20 Products

Product Documents for Cdc20 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cdc20 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cdc20 Antibody

There are currently no reviews for this product. Be the first to review Cdc20 Antibody and earn rewards!

Have you used Cdc20 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies