Cdc23 Antibody (1F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00008697-M08

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F3

Format

Azide and BSA Free
View all Cdc23 Primary Antibodies »

Product Specifications

Immunogen

CDC23 (NP_004652.1, 491 a.a. ~ 591 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP

Specificity

Reacts with cell division cycle 23 homolog (S. cerevisiae).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cdc23 Antibody (1F3) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Cdc23 Antibody (1F3) [H00008697-M08]

Immunocytochemistry/ Immunofluorescence: Cdc23 Antibody (1F3) [H00008697-M08]

Immunocytochemistry/Immunofluorescence: Cdc23 Antibody (1F3) [H00008697-M08] - Analysis of monoclonal antibody to CDC23 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Cdc23 Antibody (1F3) [H00008697-M08] - Detection limit for recombinant GST tagged CDC23 is 3 ng/ml as a capture antibody.

Applications for Cdc23 Antibody (1F3) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cdc23

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq]

Alternate Names

ANAPC8anaphase promoting complex subunit 8, Anaphase-promoting complex subunit 8, APC8CDC23 (cell division cycle 23, yeast, homolog), cell division cycle 23 homolog (S. cerevisiae), cell division cycle protein 23 homolog, CUT23, Cyclosome subunit 8

Entrez Gene IDs

8697 (Human)

Gene Symbol

CDC23

UniProt

NP_004652 (Human)

Additional Cdc23 Products

Product Documents for Cdc23 Antibody (1F3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cdc23 Antibody (1F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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