Human CDK2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-75170

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human CDK2 in samples. No significant cross-reactivity or interference between Human CDK2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.71%

Inter-Assay Precision (Precision between assays) CV% 4.78%

Recovery for Human CDK2 ELISA Kit (Colorimetric)

Recovery

86-107%

Linearity

Human CDK2 ELISA Kit (Colorimetric)

Scientific Data Images for Human CDK2 ELISA Kit (Colorimetric)

Human CDK2 ELISA Kit (Colorimetric)

ELISA: Human CDK2 ELISA Kit (Colorimetric) [NBP2-75170] -

ELISA: Human CDK2 ELISA Kit (Colorimetric) [NBP2-75170] - Standard Curve Reference

Kit Contents for Human CDK2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for Human CDK2 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human CDK2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail