CDK4 Antibody (5-C3-G3-R)

Novus Biologicals | Catalog # NBP3-32175

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 5-C3-G3-R expressed in HEK293
View all CDK4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human CDK4 aa 80-303/303. (Uniprot: P11802)

Localization

Cytoplasm, Nucleus, Nucleus membrane.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDK4 Antibody (5-C3-G3-R)

CDK4 Antibody (5-C3-G3-R)

Western Blot: CDK4 Antibody (5-C3-G3-R) [NBP3-32175]

Western Blot: CDK4 Antibody (5-C3-G3-R) [NBP3-32175] - Western blot analysis of CDK4 on different lysates with Mouse anti-CDK4 antibody (NBP3-32175) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: 293T cell lysate
Lane 3: HeLa cell lysate
Lane 4: MCF7 cell lysate
Lane 5: K-562 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32175) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for CDK4 Antibody (5-C3-G3-R)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDK4

Cdk4 is encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Long Name

Cyclin-dependent Kinase 4

Alternate Names

CMM3, PSK-J3

Gene Symbol

CDK4

Additional CDK4 Products

Product Documents for CDK4 Antibody (5-C3-G3-R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CDK4 Antibody (5-C3-G3-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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