Cdk7 Antibody (BL-80-5D4)
Novus Biologicals | Catalog # NBP2-76409
Recombinant Monoclonal Antibody
![Western Blot: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]](https://resources.rndsystems.com/images/products/Cdk7-Antibody-BL-80-5D4-Western-Blot-NBP2-76409-img0001.jpg "Western Blot: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]")
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Key Product Details
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BL-80-5D4
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Product Specifications
Immunogen
Immunogen sequence between amino acids 300 and 3416 (C-Term)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cdk7 Antibody (BL-80-5D4)
Western Blot: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]
Western Blot: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 by WB of HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and HCT 116 lysate. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG.![Immunocytochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]](https://resources.rndsystems.com/images/products/Cdk7-Antibody-BL-80-5D4-Immunocytochemistry-NBP2-76409-img0004.jpg "Immunocytochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]")
Immunocytochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]
Immunocytochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 in FFPE breast carcinoma cell line MCF-7 by ICC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.![Immunohistochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]](https://resources.rndsystems.com/images/products/Cdk7-Antibody-BL-80-5D4-Immunohistochemistry-NBP2-76409-img0003.jpg "Immunohistochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]")
Immunohistochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]
Immunohistochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 in FFPE human ovarian carcinoma by IHC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.![Flow Cytometry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]](https://resources.rndsystems.com/images/products/Cdk7-Antibody-BL-80-5D4-Flow-Cytometry-NBP2-76409-img0005.jpg "Flow Cytometry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]")
Flow Cytometry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]
Flow Cytometry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-3D4] NBP2-76409 (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.![Immunoprecipitation: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]](https://resources.rndsystems.com/images/products/Cdk7-Antibody-BL-80-5D4-Immunoprecipitation-NBP2-76409-img0002.jpg "Immunoprecipitation: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]")
Immunoprecipitation: Cdk7 Antibody (BL-80-5D4) [NBP2-76409]
Immunoprecipitation: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409).Applications for Cdk7 Antibody (BL-80-5D4)
Application
Recommended Usage
Immunohistochemistry
1:25 - 1:100
Immunoprecipitation
20 ul/0.5 mg lysate
Western Blot
1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: Cdk7
Long Name
Cyclin-dependent kinase 7
Alternate Names
CDKN7, MO15, p39 Mo15, STK1
Gene Symbol
CDK7
Additional Cdk7 Products
Product Documents for Cdk7 Antibody (BL-80-5D4)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cdk7 Antibody (BL-80-5D4)
This Product sold under License from Bethyl Laboratories, Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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