Cytokine-induced killer (CIK) cells exhibit phenotypic and functional similarities to both T cells and Natural Killer (NK) cells. While CIK cells express CD3 and expand readily in culture like T cells, they do not require functional priming for in vivo activity, a feature shared with NK cells. CIK cells consist of a heterogeneous population, including CD3+CD56-, CD3+CD56+, and CD3-CD56+ cells. Importantly, CD3+CD56+ have the most potent cytotoxic function among the CIK cells and express a number of cytokines, including IL-2, IFN-γ, TNF-α, and GM-CSF. The advantages that CIK cells have over other routes of cell therapy include their ease of in vitro propagation and their ability to be primed without the need for exogenous administration of IL-2. Over the past decade, cell therapy using CIK cells has emerged as an active area of research for the treatment of various types of cancer, such as hepatocellular carcinoma, non-small cell lung cancer, renal cell carcinoma, and gastric cancer. The ability to expand CIK cell populations ex vivo is valuable to researchers who require CIK cells for downstream applications including studies for cell therapy and cancer immunotherapy.
This kit contains the following reagents for the ex vivo expansion of human CIK cells.
Store the unopened kit at < -20 °C. Do not use past the kit expiration date.
*Provided this is within the expiration date of the kit.
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
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Flow Cytometry Analysis of Expanded Human Cytokine-induced Killer Cells. Human PBMCs were cultured ex vivo for 21 days to expand Cytokine-induced Killer (CIK) cells using reagents included in the CellXVivo™ Human CIK Cell Expansion Kit. Compared to unexpanded PBMCs (A), PBMCs treated with the kit (B) show an increased number of CD3+CD56+ CIK cells. Flow cytometric analysis was performed on day 21 of the expansion and cells were stained with Human NCAM-1/CD56 PE-conjugated Antibody, and Human CD3ε PerCP-conjugated Antibody. Quadrants were set based on isotope-stained samples.
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Kit-expanded CIK Cells are Cytotoxic to Tumor Cells. Cytokine-induced Killer (CIK) cells expanded using the CellXVivo™ CIK Cell Expansion Kit were assessed for toxicity against the killer cell-sensitive K562 tumor cell line. K562 cell were loaded with the live-cell dye, Calcein-AM (Tocris®, Catalog # 5119) prior to being mixed with CIK cells for 4 hours at the indicated effector-to-target cell (E:T) ratios (A). (B) Graph showing % killing of tumor cells by CIK cells at the indicated E:T ratios as determined by measuring relative fluorescence intensity of released Calcein-AM.
Refer to the product datasheet for complete product details.
Protocol for CIK Cell Expansion using the CellXVivo™ Human CIK Cell Expansion Kit.
Isolate PBMCs from human blood.
Perform a cell count.
Suspend 1 x 106 PBMCs/mL in Human CIK Cell Expansion Media. Culture the cells in the pre-coated 75 cm2 flask for a total of 14 days.
Culture the cells for a total of 21 days.
Refresh media and split cells on day 6, 12, and 18.
Verify expanded NK cells by analyzing NK cell marker expression via flow cytometry (optional).
CIK cells are ready to be used for downstream applications.
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