CENPB Antibody

Novus Biologicals | Catalog # NB100-2369

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CENPB Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 540 and the C-terminus (residue 599) of human Centromere Protein B using the numbering given in entry SwissProt entry P07199 (GeneID 1059).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CENPB Antibody

Immunocytochemistry/ Immunofluorescence: CENPB Antibody [NB100-2369]

Immunocytochemistry/ Immunofluorescence: CENPB Antibody [NB100-2369]

Immunocytochemistry/Immunofluorescence: CENPB Antibody [NB100-2369] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-B used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
Immunocytochemistry/ Immunofluorescence: CENPB Antibody [NB100-2369]

Immunocytochemistry/ Immunofluorescence: CENPB Antibody [NB100-2369]

Immunocytochemistry/Immunofluorescence: CENPB Antibody [NB100-2369] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-B used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.

Applications for CENPB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500
Application Notes
Formaldehyde-Fixed Cells permeabilized with Triton-X 100 is recommended. Likely to work with frozen sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CENPB

This gene product is a highly conserved protein associated with the centromere. It is a DNA-binding protein containing a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Alternate Names

CENP-B, centromere autoantigen B, Centromere protein B, centromere protein B (80kD), centromere protein B, 80kDa, major centromere autoantigen B

Entrez Gene IDs

1059 (Human)

Gene Symbol

CENPB

UniProt

P07199 (Human)

Additional CENPB Products

Product Documents for CENPB Antibody

Certificate of Analysis

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Product Specific Notices for CENPB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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