Ceruloplasmin Antibody (JE59-01)

Novus Biologicals | Catalog # NBP3-32182

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE59-01 expressed in HEK293
View all Ceruloplasmin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide. (Uniprot: P13635)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ceruloplasmin Antibody (JE59-01)

Ceruloplasmin Antibody (JE59-01)

Western Blot: Ceruloplasmin Antibody (JE59-01) [NBP3-32182]

Western Blot: Ceruloplasmin Antibody (JE59-01) [NBP3-32182] - Western blot analysis of Ceruloplasmin on different lysates with Rabbit anti-Ceruloplasmin antibody (NBP3-32182) at 1/1,000 dilution.

Lane 1: Rat liver tissue lysate
Lane 2: Rat testis tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 122 kDa
Observed band size: 140 kDa

Exposure time: 3 minutes 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32182) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Ceruloplasmin Antibody (JE59-01)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ceruloplasmin

The protein encoded by the Ceruloplasmin gene is a metalloprotein that binds most of the copper in plasma and is involved in theperoxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, whichresults in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities.(provided by RefSeq)

Alternate Names

ceruloplasmin, ceruloplasmin (ferroxidase), CP-2, EC 1.16.3.1, Ferroxidase

Gene Symbol

CP

Additional Ceruloplasmin Products

Product Documents for Ceruloplasmin Antibody (JE59-01)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ceruloplasmin Antibody (JE59-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Ceruloplasmin Antibody (JE59-01)

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  • Q: We want to set up a Ceruloplasmin enzyme immunoassay. Could you suggest a suitable/optimal antibody pair for a sandwich format?

    A: Currently we do not carry any validated antibody pairs for sandwich ELISA to detect ceruloplasmin, however we do have ELISA kits that have been optimized and validated for human and rat.

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