Q: We want to set up a Ceruloplasmin enzyme immunoassay. Could you suggest a suitable/optimal antibody pair for a sandwich format?

A: Currently we do not carry any validated antibody pairs for sandwich ELISA to detect ceruloplasmin, however we do have ELISA kits that have been optimized and validated for human and rat.

Mouse Ceruloplasmin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82160

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse Ceruloplasmin in samples. No Significant cross-reactivity or interference between Mouse Ceruloplasmin and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 6%

Inter-Assay Precision (Precision between assays) CV% 5%

Recovery for Mouse Ceruloplasmin ELISA Kit (Colorimetric)

Recovery

average = 90% for serum; average = 97% for EDTA plasma; average = 99% for cell culture media

Linearity

Scientific Data Images for Mouse Ceruloplasmin ELISA Kit (Colorimetric)

ELISA: Mouse Ceruloplasmin ELISA Kit (Colorimetric) [NBP2-82160] -

ELISA: Mouse Ceruloplasmin ELISA Kit (Colorimetric) [NBP2-82160] - Standard Curve Reference

Kit Contents for Mouse Ceruloplasmin ELISA Kit (Colorimetric)

Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Ceruloplasmin

The protein encoded by the Ceruloplasmin gene is a metalloprotein that binds most of the copper in plasma and is involved in theperoxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, whichresults in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities.(provided by RefSeq)

Alternate Names

ceruloplasmin, ceruloplasmin (ferroxidase), CP-2, EC 1.16.3.1, Ferroxidase

Gene Symbol

Additional Ceruloplasmin Products

Product Documents for Mouse Ceruloplasmin ELISA Kit (Colorimetric)

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Certificate of Analysis

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Product Specific Notices for Mouse Ceruloplasmin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Mouse Ceruloplasmin ELISA Kit (Colorimetric)

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