CHAC1 Antibody (OTI1E2)

Novus Biologicals | Catalog # NBP2-46273

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E2
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Product Specifications

Immunogen

Full length human recombinant protein of human CHAC1(NP_077016) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHAC1 Antibody (OTI1E2)

Western Blot: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Western Blot: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Western Blot: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAC1.
Immunocytochemistry/ Immunofluorescence: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunocytochemistry/ Immunofluorescence: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunocytochemistry/Immunofluorescence: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CHAC1.
Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273]

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for CHAC1 Antibody (OTI1E2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:4000

Reviewed Applications

Read 1 review rated 3 using NBP2-46273 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHAC1

Alternate Names

cation transport regulator-like protein 1, ChaC, cation transport regulator homolog 1 (E. coli)

Gene Symbol

CHAC1

Additional CHAC1 Products

Product Documents for CHAC1 Antibody (OTI1E2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CHAC1 Antibody (OTI1E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CHAC1 Antibody (OTI1E2)

Customer Reviews for CHAC1 Antibody (OTI1E2) (1)

3 out of 5
1 Customer Rating
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  • CHAC1 Antibody (OTI1E2)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Huh7 cell lysate
    Species: Human
    Verified Customer | Posted 02/01/2019
    Cell lysates loaded in increasing protein concentrations from 30 to 50 microgram
    CHAC1 Antibody (OTI1E2) NBP2-46273

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Protocols

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