CHD8 Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP2-58582PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

Applications

Antibody Competition
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Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human CHD8.

Source: E. coli

Amino Acid Sequence: PEFAVDPRFLAYMEDRRKQKWQRCKKNNKAELNCLGMEPVQTANSRNGKKGHHTETVFNRVLPGPIAPESSKKRARRMRPDLSKMMALMQGGSTGSLSLHNTFQHSSSGLQSVSSLGHSSATSASLPFMPFVMGGAPSSPHVD

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-58582.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP2-58582PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: CHD8

CHD8 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD8 has been reported to interact with duplin, a protein involved in the wnt signaling pathway, and CTCF, a protein involved in heterochromatin formation.

Alternate Names

ATP-dependent helicase CHD8, CHD-8, chromodomain helicase DNA binding protein 8, DKFZp686N17164, duplin, EC 3.6.1, EC 3.6.1.7, EC 3.6.4.12, Helicase with SNF2 domain 1chromodomain-helicase-DNA-binding protein 8, HELSNF1, KIAA1564axis duplication inhibitor

Gene Symbol

CHD8

Additional CHD8 Products

Product Documents for CHD8 Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CHD8 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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FAQs for CHD8 Recombinant Protein Antigen

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  • Q: I see that all of your CHD8 antibodies say that they are specific for human. Have any been tested in mouse? I want to know if they have and were found not to be suitable. Ideally, I would like to try one in mouse for immunocytochemistry.

    A:

    We have not tested any of our CHD8 antibodies in mouse samples, and do not have any data to support whether they will work for you or not. If you would like to purchase one to try on your samples, you will qualify for our Innovators Reward Program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data.

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