Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25411

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2479 expressed in HEK293

Format

Azide and BSA Free
View all Chikungunya Virus nsp3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of Chikungunya Virus nsp3.

Reactivity Notes

Chikungunya Virus

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free

Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free

Western Blot: Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free [NBP3-25411] -

Western Blot: Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free [NBP3-25411] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chikungunya virus nsP3 antibody [HL2479] (NBP3-25411) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free

Immunohistochemistry-Paraffin: Chikungunya Virus nsp3 Antibody (HL2479) [NBP3-25411] -

Chikungunya virus nsP3 antibody [HL2479] detects Chikungunya virus nsP3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Chikungunya virus nsP3 - transfected 293T cell pellet.
Green: Chikungunya virus nsP3 stained by Chikungunya virus nsP3 antibody [HL2479] (NBP3-25411) diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Chikungunya Virus nsp3 Antibody (HL2479) [NBP3-25411] -

Immunofluorescent analysis of Chikungunya virus infected cells using Chikungunya virus nsP3 antibody [HL2479] antibody (NBP3-25411).
Sample: Multiple virus infected cells slide.
Green: Chikungunya virus nsP3 antibody [HL2479] antibody (NBP3-25411) diluted at 1:100.
Blue: Fluoroshield with DAPI.
Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Chikungunya Virus nsp3 Antibody (HL2479) [NBP3-25411] -

Chikungunya virus nsP3 antibody [HL2479] detects Chikungunya virus nsP3 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: Chikungunya virus nsP3 stained by Chikungunya virus nsP3 antibody [HL2479] (NBP3-25411) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Scale bar= 10um.

Applications for Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:5000-1:50000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chikungunya Virus nsp3

Chikungunya virus (CHIKV) belongs to the family of alphaviruses and is transmitted by Aedes mosquitoes resulting in infection characterized by symptoms including arthritis-like joint pain and inflammation, fever, rash, and headache (1-3). The CHIKV genome consists of an 11.8 kilobase (kb) positive-sense, single-stranded RNA virus that has two open reading frames (ORFs) (1-3). The first ORF at the 5' end encodes a 2472 amino acid (aa) nonstructural polyprotein and the ORF at the 3' end encodes a 1244 aa structural polyprotein (1-3). The polyproteins generate four nonstructural proteins (nsP1-4) and five structural proteins: capsid (C), envelope E1, E2, E3, and 6K (1-3). The nonstructural proteins comprise CHIKV's RNA replicase (1,2). Structurally, CHIKV has a diameter of ~65 nm and the virion is formed by an icosahedral nucleocapsid shell encapsulating genomic RNA and surrounded by a lipid bilayer envelope (2,3).

Although analysis suggests CHIKV originated in Africa over 500 years ago, first infections weren't reported until the 1950s (1-3). CHIKV has evolved three distinct genotypes, or strains, based on location, termed West African (WA), East/Central/Southern African (ECSA), and Asian (2-3). The WA strain has been most closely associated with enzootic transmission whereas the ECSA strain contributes more to urban epidemics (2). Nonhuman primates are the primary reservoir for the viral host with transmission occurring via mosquitos biting and infecting humans (1-3). Upon acute infection, the virus replicates in cells including fibroblasts and macrophages resulting in innate immune response in infected tissues characterized by infiltrating cells like macrophages, monocytes, natural killer (NK) cells, and lymphocytes (2,3). Infection results in increased production of proinflammatory cytokines such as interferon alpha (IFN-alpha) and interleukin 6 (IL-6), chemokines, and growth factors (2,3). Physical manifestations of infection are high fever, polyarthralgia, headache, arthritis, and rash (1-3). CHIKV symptoms can be confused with other infections like those from dengue fever and Zika virus (1-3). There are no specific antivirals or vaccines for CHIKV, but rather symptoms are treated with antipyretics and non-steroidal anti-inflammatory drugs (NSAIDs) (2,3). While in vitro culture models and in vivo rodent and non-human primate models have been used to study CHIKV pathogenesis and advance our knowledge of the disease, the specific cellular mechanisms are not fully understood (3).

References

1. Vu DM, Jungkind D, Angelle Desiree LaBeaud. Chikungunya Virus. Clin Lab Med. 2017;37(2):371-382. https://doi.org/10.1016/j.cll.2017.01.008

2. Silva LA, Dermody TS. Chikungunya virus: epidemiology, replication, disease mechanisms, and prospective intervention strategies. J Clin Invest. 2017;127(3):737-749. https://doi.org/10.1172/JCI84417

3. Ganesan VK, Duan B, Reid SP. Chikungunya Virus: Pathophysiology, Mechanism, and Modeling. Viruses. 2017;9(12):368. Published 2017 Dec 1. https://doi.org/10.3390/v9120368

Alternate Names

CHIKV nsp3, Non-structural protein 3

Additional Chikungunya Virus nsp3 Products

Product Documents for Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Chikungunya Virus nsp3 Antibody (HL2479) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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