Recombinant Cholera Toxin Beta Protein

Name: Recombinant Cholera Toxin Beta Protein
Brand: Novus Biologicals
SKU: NBP2-61449
Price: 228 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-61449

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Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Applications

SDS-PAGE

View all Cholera Toxin Beta Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 103 amino acids corresponding to Cholera Toxin Beta Source: E. coli

Uniprot ID: P01556

Amino Acid Sequence: TPQNITDLCA EYHNTQIYTL NDKIFSYTES LAGKREMAII TFKNGAIFQV EVPGSQHIDS QKKAIERMKD TLRIAYLTEA KVEKLCVWNN KTPHAIAAIS MAN

Purity

>98%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Cholera Toxin Beta as determined by LAL method.

Predicted Molecular Mass

11.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Cholera Toxin Beta Protein

SDS-PAGE: Recombinant Cholera Toxin Beta Protein [NBP2-61449]

SDS-Page: Recombinant Bacterial Cholera Toxin Beta Protein [NBP2-61449] - Recombinant Bacteria, Bacterial Cholera Toxin Beta Protein [NBP2-61449] - Recombinant Bacteria, Viral Cholera Toxin Beta Protein [NBP2-61449] - Recombinant Bacteria Cholera Toxin Beta Protein [NBP2-61449]

Formulation, Preparation, and Storage

NBP2-61449

Formulation	0.2 um filtered solution in 5 mM PB, pH 7.0, 75 mM NaCl.
Preservative	50% glycerol
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: Cholera Toxin Beta

The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.

Alternate Names

Cholera enterotoxin B chain, Cholera enterotoxin beta chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Cholera toxin B protein, Choleragenoid, CTX B, CTXB, TOX B, toxB, VC1456

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Product Specific Notices for Recombinant Cholera Toxin Beta Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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