Chroman 1

Tocris Bioscience | Catalog # 7163

Highly potent and selective ROCK 2 inhibitor; improves cell survival after cryogenesis
Tocris Bioscience

Key Product Details

Description

Highly potent and selective ROCK 2 inhibitor; improves cell survival after cryogenesis
View all Rho-kinase Inhibitors »

Product Description

Chroman 1 is a highly potent and selective ROCK2 (Rho-kinase) inhibitor (IC50 values are 1 and 52 pM at ROCK2 and ROCK1, respectively). Chroman 1 exhibits >2000-fold selectivity for ROCK2 over a range of related kinases including MRCK, PKA, and AKT1 (IC50 values are 150, >20000, and >20000 nM, respectively). Chroman 1 promotes survival of pluripotent stem cells (PSCs) in culture when used in combination with Emricasan (Cat. No. 7310), Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), and trans-ISRIB (Cat. No. 5284), a combination known as CEPT. When used as part of the CEPT cocktail, Chroman 1 also improves survival of differentiated cells following cryopreservation with cardiomyocyte cell survival increasing by 36% and motor neuron survival improving by 63% compared with DMSO controls.

Product Specifications for Chroman 1

Molecular Weight

509.43

Formula

C24H28N4O4.2HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(3S)-N-[2-[2-(Dimethylamino)ethoxy]-4-(1H-pyrazol-4-yl)phenyl]-3,4-dihydro-6-methoxy-2H-1-benzopyran-3-carboxamide dihydrochloride

InChI Key

ROFMCPHQNWGXGE-SFHVURJKSA-N

SMILES

COC1=CC=C2OC[C@@H](C(NC3=C(C=C(C4=CNN=C4)C=C3)OCCN(C)C)=O)CC2=C1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 2.55 5
DMSO 50.94 100

Preparing Stock Solutions for Chroman 1

The following data is based on the product molecular weight 509.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.96 mL 9.81 mL 19.63 mL
5 mM 0.39 mL 1.96 mL 3.93 mL
10 mM 0.20 mL 0.98 mL 1.96 mL
50 mM 0.04 mL 0.20 mL 0.39 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 20 publications citing the usage of this product.

Product Documents for Chroman 1

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Chroman 1

For research use only

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