Catalog # Availability Size / Price Qty
trans-ISRIB | CAS No. 1597403-47-8 | Other ER Stress/UPR Activators
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Description: Integrated stress response (ISR) inhibitor

Chemical Name: N,N'-trans-1,4-cyclohexanediylbis[2-(4-chlorophenoxy)acetamide]

Purity: ≥99%

Product Details
Citations (14)
Reviews (1)

Biological Activity

trans-ISRIB is a potent integrated stress response (ISR) inhibitor; reverses the effects of eIF2α phosphorylation (IC50 = 5 nM) and restores cell translation capacity. Acts downstream of PERK, targeting interactions between eIF2α kinases and elF2B. Blocks ATF4 production without altering the IRE1 or ATF6 responses in vitro. Displays memory enhancement in hippocampus-dependent spatial and fear-associated learning in rodents. trans-ISRIB promotes survival of pluripotent stem cells in culture when used in combination with Chroman 1 (Cat. No. 7163), Emricasan (Cat. No. 7310), and Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), a combination known as CEPT. When used as part of the CEPT cocktail, trans-ISRIB improves survival of differentiated cells following cryopreservation. Brain penetrant.

Technical Data

Soluble to 10 mM in DMSO with gentle warming
Store at +4°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Inhibiting checkpoint kinase 1 protects bone from bone resorption by mammary tumor in a mouse model
    S Liu, Y Liu, K Minami, A Chen, Q Wan, Y Yin, L Gan, A Xu, N Matsuura, M Koizumi, Y Liu, S Na, J Li, H Nakshatri, BY Li, H Yokota
    Oncotarget, 2018;9(10):9364-9378.
  2. Pharmacological brake-release of mRNA translation enhances cognitive memory.
    Sidrauski et al.
    Elife, 2013;2:e00498
  3. Stress responses. Mutations in a translation initiation factor identify the target of a memory-enhancing compound.
    Sekine et al.
    Science, 2015;348:1027
  4. Cell biology. Blocking stress response for better memory?
    Science, 2015;348:967

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Citations for trans-ISRIB

The citations listed below are publications that use Tocris products. Selected citations for trans-ISRIB include:

14 Citations: Showing 1 - 10

  1. Stress-free cell aggregation by using the CEPT cocktail enhances embryoid body and organoid fitness.
    Authors: Ryu Et al.
    Biofabrication  2023;16:015016
  2. A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas.
    Authors: Lisa D Et al.
    Cancer Discov  2023;13:766-795
  3. Polyadenylated RNA and RNA-Binding Proteins Exhibit Unique Response to Hyperosmotic Stress.
    Authors: Jeffrey D Et al.
    Front Cell Dev Biol  2022;9:809859
  4. Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR.
    Authors: Jing Et al.
    Cell Rep  2022;40:111092
  5. Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.
    Authors: Jason A Et al.
    Nat Commun  2022;13:3244
  6. Cholinergic neurons constitutively engage the ISR for dopamine modulation and skill learning in mice.
    Authors: Viviana Et al.
    Science  2021;372
  7. SARS-CoV-2 infection induces beta cell transdifferentiation.
    Authors: Bo Et al.
    Cell Metab  2021;33:1577-1591.e7
  8. Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells.
    Authors: Tristan Et al.
    Stem Cell Rep.  2021;16:3076
  9. Saturated Fatty Acids Promote GDF15 Expression in Human Macrophages through the PERK/eIF2/CHOP Signaling Pathway.
    Authors: Jacques Et al.
    Nutrients  2020;12
  10. A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells.
    Authors: Chen Et al.
    Nat.Methods  2019;18:528


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I’m vitro on mice
By Anonymous on 09/05/2021
Species: Mouse

Mouse study

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