trans-ISRIB

Tocris Bioscience | Catalog # 5284

Integrated stress response (ISR) inhibitor
Tocris Bioscience

Key Product Details

Description

Integrated stress response (ISR) inhibitor
View all Other ER Stress/UPR Activators »

Product Description

trans-ISRIB is a potent integrated stress response (ISR) inhibitor; reverses the effects of eIF2α phosphorylation (IC50 = 5 nM) and restores cell translation capacity. Acts downstream of PERK, targeting interactions between eIF2α kinases and elF2B. Blocks ATF4 production without altering the IRE1 or ATF6 responses in vitro. Displays memory enhancement in hippocampus-dependent spatial and fear-associated learning in rodents. trans-ISRIB promotes survival of pluripotent stem cells in culture when used in combination with Chroman 1 (Cat. No. 7163), Emricasan (Cat. No. 7310), and Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), a combination known as CEPT. When used as part of the CEPT cocktail, trans-ISRIB improves survival of differentiated cells following cryopreservation. Brain penetrant.

Product Specifications for trans-ISRIB

Molecular Weight

451.34

Formula

C22H24Cl2N2O4

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

N,N'-trans-1,4-cyclohexanediylbis[2-(4-chlorophenoxy)acetamide]

CAS Number

1597403-47-8

PubChem ID

1011240

InChI Key

HJGMCDHQPXTGAV-UHFFFAOYSA-N

SMILES

O=C(COC1=CC=C(Cl)C=C1)N[C@@H]2CC[C@@H](NC(COC3=CC=C(Cl)C=C3)=O)CC2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 4.51 10 with gentle warming

Preparing Stock Solutions for trans-ISRIB

The following data is based on the product molecular weight 451.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 22.16 mL 110.78 mL 221.56 mL
0.5 mM 4.43 mL 22.16 mL 44.31 mL
1 mM 2.22 mL 11.08 mL 22.16 mL
5 mM 0.44 mL 2.22 mL 4.43 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 73 publications citing the usage of this product.

Product Documents for trans-ISRIB

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for trans-ISRIB

For research use only

Customer Reviews for trans-ISRIB (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used trans-ISRIB?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • I’m vitro on mice
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Verified Customer | Posted 09/05/2021
    Mouse study
  • Inhibits integrated stress response in HEK293 cells
    Name: mandi gandelman
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 05/01/2019
    Used trans-ISRIB to inhibit the integrated stress response (ISR) in HEK293 cells. Induced the ISR and added ISRIB (100 and 500nM) overnight. Evaluated p-eif2a (unchanged) and cleaved caspase 3 (increased in a dose-dependent manner with ISRIB.
    trans-ISRIB 5284
  • High specific inhibitor
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: cortical neurons
    Verified Customer | Posted 11/01/2017
    Toxicity assay. 500 nM concentration was used. Highly effective Integrated stress response (ISR) inhibitor. Works very well.

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All