Cisplatin

Tocris Bioscience | Catalog # 2251

Potent pro-apoptotic anticancer agent; activates caspase-3
Tocris Bioscience

Key Product Details

Description

Potent pro-apoptotic anticancer agent; activates caspase-3

Alternative Names

CDDP
View all Caspase Activators »

Product Description

Cisplatin is a potent anticancer agent that blocks DNA synthesis. Induces apoptosis via p53-dependent and -independent mechanisms. Inhibits X-linked inhibitor of apoptosis protein (XIAP) expression and activates caspase-3. In certain glioma cell lines, sensitizes cells to TNF-α-induced apoptosis.

Product Specifications for Cisplatin

Molecular Weight

300.05

Formula

Cl2H6N2Pt

Storage

Store at RT

Chemical Name

cis-Diaminodichloroplatinum

CAS Number

15663-27-1

PubChem ID

84691

InChI Key

LXZZYRPGZAFOLE-UHFFFAOYSA-L

SMILES

N.N.[Cl-].[Cl-].[Pt++]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 1.5 5 with gentle warming

Preparing Stock Solutions for Cisplatin

The following data is based on the product molecular weight 300.05.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 66.66 mL 333.28 mL 666.56 mL
0.25 mM 13.33 mL 66.66 mL 133.31 mL
0.5 mM 6.67 mL 33.33 mL 66.66 mL
2.5 mM 1.33 mL 6.67 mL 13.33 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 178 publications citing the usage of this product.

Product Documents for Cisplatin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cisplatin

For research use only

⚠ WARNING: This product can expose you to chemicals including Cisplatin, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov

Customer Reviews for Cisplatin (7)

5 out of 5
7 Customer Ratings
5 Stars
100%
4 Stars
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2 Stars
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1 Stars
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Customer Images

Showing  1 - 5 of 7 reviews Showing All
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  • Worked well
    Name: Kishor Pant
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HepG2 and Huh
    Verified Customer | Posted 03/24/2023
    Please always try to use the fresh sample
    We used this in a cell proliferation assay in HepG2 and other liver cancer cell lines for 24-48 hours and always worked well. The graph shows % proliferation (x) vs increasing concentration of cisplatin (0 - 20 uM) (y).
    Cisplatin 2251
  • Good Product
    Name: Kumar Gupta
    Species: Human
    Assay Type: Ex Vivo
    Cell Line/Tissue: Jurkat Cells
    Verified Customer | Posted 04/04/2021
    Jurkat cells were treated with 20 μM cisplatin. Apoptosis data is attached.
    Cisplatin 2251
  • Cytotoxicity of Cisplatin in B16F10
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: B16F10
    Verified Customer | Posted 01/22/2020
    B16F10 Cell were treated with 200 mM of Cisplatin for 18 hours and Cells viability was assessed using Flowcytometery
    Cisplatin 2251
  • CIPN model in C57Bl/6J mice
    Name: Chris Holton
    Species: Mouse
    Assay Type: In Vivo
    Verified Customer | Posted 01/17/2020
    Administration in mice chemotherapy-induced-peripheral neuropathy model. Cisplatin (2.3mg/kg) dissolved in sterile saline and administered IP (10ml/kg) daily dose for 5 days, followed by 5 days of rest and then a second round of daily dosing for 5 days. Hindpaw mechanical hypersensitivity for at least 15 days after.
    Cisplatin 2251
  • Paclitaxel, docetaxel, vincristine, vinblastine, colchicine, cisplatin, and mitoxantrone screen for Human epidermal carcinoma
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Human epidermal carcinoma cell line
    Verified Customer | Posted 11/17/2019
    Human epidermal carcinoma cell line was treated with Paclitaxel, docetaxel, vincristine, vinblastine, colchicine, cisplatin, and mitoxantrone which purchased from Tocris Bioscience.
    Cisplatin 2251
  • works perfectly
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: A549
    Verified Customer | Posted 07/28/2019
    Used this product in caspase assay.
  • dose response
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: H524
    Verified Customer | Posted 05/01/2018
    Determined the optimal dose to identify the chemoresistant population in our cell line. Cells were treated with escalating doses of cisplatin (0.01-100uM) and viability determined 4 days post treatment.
    Cisplatin 2251

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