ClariTSA™ Fluorophore Kit 520
Tocris Bioscience | Catalog # 7523
Key Product Details
Description
Product Description
Please note: this product was formerly known as TSA Vivid Fluorophore Kit 520.
ClariTSA™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. ClariTSA reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.
The basis for signal amplification comes from the specific and high-density deposition of ClariTSA dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).
Excitation maximum = 494 nm; emission maximum = 517 nm
Please see the product datasheet or protocol for information on product use.
ClariTSA™ is a trademark of Bio-Techne Corp.
Scientific Data Images for ClariTSA™ Fluorophore Kit 520
Application of ClariTSA™ Fluorophore Kit 520 in RNAscope Multiplex Fluorescent v2 Assay
RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and ClariTSA™ Fluorophore Kit 520 dye (right). Dye used at 1:1500 dilution. Marker shown is Polr2a in green. Nuclei are counterstained with DAPI (blue, Cat. No. 5748).
Optical Data
 |
Emission Color | Green |
| λabs | 494 nm | |
| λem | 517 nm |
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Product Specifications for ClariTSA™ Fluorophore Kit 520
Storage
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 15 publications citing the usage of this product.
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Product Documents for ClariTSA™ Fluorophore Kit 520
Certificate of Analysis
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Product Specific Notices for ClariTSA™ Fluorophore Kit 520
For research use only
Citations for ClariTSA™ Fluorophore Kit 520
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