ClariTSA™ Fluorophore Kit 650

Tocris Bioscience | Catalog # 7527

Signal amplification kit for use in ISH, ICC and IHC
Tocris Bioscience
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Key Product Details

Description

Signal amplification kit for use in ISH, ICC and IHC

Product Description

Please note: this product was formerly known as TSA Vivid Fluorophore Kit 650.

ClariTSA™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. ClariTSA reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.

The basis for signal amplification comes from the specific and high-density deposition of ClariTSA dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).

Excitation maximum = 654 nm; emission maximum = 668 nm

Please see the product datasheet or protocol for information on product use.

ClariTSA™ is a trademark of Bio-Techne Corp.

Scientific Data Images for ClariTSA™ Fluorophore Kit 650

Application of ClariTSA™ Fluorophore Kit 650 in RNAscope Multiplex Fluorescent v2 Assay

Application of ClariTSA™ Fluorophore Kit 650 in RNAscope™ Multiplex Fluorescent v2 Assay.

RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and ClariTSA™ Fluorophore Kit 650 (right). Dye used at 1:1500 dilution. Marker shown is UBC in white. Nuclei are counterstained with DAPI (blue, Catalog # 5748, Tocris).

Optical Data

Spectral data for ClariTSA Fluorophore Kit 650 Emission Color Red
λabs 654 nm
λem 668 nm

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Product Specifications for ClariTSA™ Fluorophore Kit 650

Storage

-20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Calculators

Calculate the dilution required to prepare a stock solution.

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 24 publications citing the usage of this product.

There are currently no references for this product.

Product Documents for ClariTSA™ Fluorophore Kit 650

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ClariTSA™ Fluorophore Kit 650

For research use only

Citations for ClariTSA™ Fluorophore Kit 650

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