ClariTSA™ Fluorophore Kit 650
Tocris Bioscience | Catalog # 7527
Key Product Details
Description
Product Description
Please note: this product was formerly known as TSA Vivid Fluorophore Kit 650.
ClariTSA™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. ClariTSA reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.
The basis for signal amplification comes from the specific and high-density deposition of ClariTSA dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).
Excitation maximum = 654 nm; emission maximum = 668 nm
Please see the product datasheet or protocol for information on product use.
ClariTSA™ is a trademark of Bio-Techne Corp.
Scientific Data Images for ClariTSA™ Fluorophore Kit 650
Application of ClariTSA™ Fluorophore Kit 650 in RNAscope™ Multiplex Fluorescent v2 Assay.
RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and ClariTSA™ Fluorophore Kit 650 (right). Dye used at 1:1500 dilution. Marker shown is UBC in white. Nuclei are counterstained with DAPI (blue, Catalog # 5748, Tocris).
Optical Data
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Emission Color | Red |
| λabs | 654 nm | |
| λem | 668 nm |
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Product Specifications for ClariTSA™ Fluorophore Kit 650
Storage
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Background References
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Product Documents for ClariTSA™ Fluorophore Kit 650
Certificate of Analysis
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Product Specific Notices for ClariTSA™ Fluorophore Kit 650
For research use only
Citations for ClariTSA™ Fluorophore Kit 650
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