ClariTSA™ Fluorophore Kit 690
Tocris Bioscience | Catalog # 9007
Key Product Details
Description
Product Description
ClariTSA™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. ClariTSA reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.The basis for signal amplification comes from the specific and high-density deposition of ClariTSA dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).
Excitation maximum = 671 nm; emission maximum = 684 nm
Please see the product datasheet or protocol for information on product use.
ClariTSA™ is a trademark of Bio-Techne Corp.
Scientific Data Images for ClariTSA™ Fluorophore Kit 690
Application of ClariTSA™ Fluorophore Kit 690 in RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells.
The target visualised in the image is HPRT1 (red, 1:1750 dilution, 690 channel, ClariTSA™ 690 kit); DAPI (dark blue, Cat. No. 5748) was used as a nuclear counterstain. Imaging was conducted using an Akoya PhenoImager with standardised imaging conditions.
Application of ClariTSA™ Fluorophore Kit 690 in RNAscope™ Multiplex Fluorescent v2 Assay in human colon tissue.
The target visualised in the image is HPRT1 (red, 1:750 dilution, 690 channel, ClariTSA™ 690 kit); DAPI (dark blue, Cat. No. 5748) was used as a nuclear counterstain. Imaging was conducted using an Akoya PhenoImager with standardised imaging conditions.
Product Specifications for ClariTSA™ Fluorophore Kit 690
Storage
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Background References
References are publications that support the biological activity of the product.
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Product Documents for ClariTSA™ Fluorophore Kit 690
Certificate of Analysis
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Product Specific Notices for ClariTSA™ Fluorophore Kit 690
For research use only
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