Clathrin Heavy Chain 1/CHC17 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30732

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Clathrin Heavy Chain 1/CHC17 Primary Antibodies »

Product Specifications

Immunogen

Between 1625 and 1675

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Clathrin Heavy Chain 1/CHC17 Antibody - BSA Free

Clathrin Heavy Chain 1/CHC17 Antibody

Immunoprecipitation:[NBP3-30732]-

Immunoprecipitation:[NBP3-30732]-Detection of human CHC by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHC antibody used for IP at 6 µg per reaction. CHC was also immunoprecipitated by rabbit anti-CHC antibody. For blotting immunoprecipitated CHC, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for Clathrin Heavy Chain 1/CHC17 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Clathrin Heavy Chain 1/CHC17

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self assembles into a polyhedral cytoskeletal element. Self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Long Name

Clathrin Heavy Chain on Chromosome 17

Alternate Names

CHC17, CLH17, CLTC, CLTCL2

Gene Symbol

CLTC

Additional Clathrin Heavy Chain 1/CHC17 Products

Product Documents for Clathrin Heavy Chain 1/CHC17 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Clathrin Heavy Chain 1/CHC17 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail