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Rat Claudin-4 ELISA Kit (Colorimetric)

Name: Rat Claudin-4 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-54847

Novus Biologicals | Catalog # NBP3-54847

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Product Documents

Product Specifications
Precision
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.063 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Kit Contents for Rat Claudin-4 ELISA Kit (Colorimetric)

Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Claudin-4

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Alternate Names

Claudin4, CLDN4, CPE-R, CPETR1, WBSCR8

Gene Symbol

CLDN4

Additional Claudin-4 Products

Product Documents for Rat Claudin-4 ELISA Kit (Colorimetric)

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Certificate of Analysis

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Product Specific Notices for Rat Claudin-4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.