Human Claudin-5 ELISA Kit (Colorimetric)

Name: Human Claudin-5 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-75332
Price: 749 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-75332

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Product Documents

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human CLDN5 in samples. No significant cross-reactivity or interference between Human CLDN5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.82%

Inter-Assay Precision (Precision between assays) CV% 5.35%

Recovery for Human Claudin-5 ELISA Kit (Colorimetric)

Recovery

93-109%

Linearity

Scientific Data Images for Human Claudin-5 ELISA Kit (Colorimetric)

ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] -

ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] - Standard Curve Reference

Kit Contents for Human Claudin-5 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Claudin-5

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrome, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.The human claudin-5 gene maps to chromosome 22q11.21.

Alternate Names

AWAL, BEC1, Claudin5, CLDN5, CPETRL1, TMDVCF, TMVCF1

Gene Symbol

CLDN5

Additional Claudin-5 Products

Product Documents for Human Claudin-5 ELISA Kit (Colorimetric)

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Download Manual - Human Claudin-5 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Claudin-5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.