Claudin-6 Antibody (9H8) - BSA Free

Name: Claudin-6 Antibody (9H8) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26651
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-26651

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Cynomolgus Monkey

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG₁ Clone # 9H8 expressed in HEK293

Format

BSA Free

View all Claudin-6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant Human Claudin-6 protein [UniProt P56747]

Clonality

Monoclonal

Host

Human

Isotype

IgG₁

Scientific Data Images for Claudin-6 Antibody (9H8) - BSA Free

ELISA: Claudin-6 Antibody (9H8) [NBP3-26651] -

ELISA: Claudin-6 Antibody (9H8) [NBP3-26651] - The Binding Activity of Human Claudin-6 with Claudin-6 Antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Claudin-6 at 10 ug/mL can bind Claudin-6 Antibody, the EC50 is 1.501-2.035 ng/mL.

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651] -

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651] - Untransfected HEK293T cells (green line) and transfected Human CLDN6 HEK293T stable cells (red line) were stained with anti-CLDN6/9 recombinant antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651]

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651] -Untransfected CHO-K1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

ELISA: Claudin-6 Antibody (9H8) [NBP3-26651]

ELISA: Claudin-6 Antibody (9H8) [NBP3-26651] -The Binding Activity of Macaca fascicularis CLDN6 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN6 at 5 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀ is 4.186-6.635 ng/mL.
The VLPs (CSB-MP3838) is negative control.

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651]

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651] -Untransfected MC38 cells (green line) and transfected Human CLDN9 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2µg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651]

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651] -Untransfected MC38 cells (green line) and transfected Human CLDN6 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2µg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

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Applications for Claudin-6 Antibody (9H8) - BSA Free

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% Glycerol, 0.01M PBS, PH 7.4

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Claudin-6

The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and junction adhesion molecule. Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-6 is expressed in differentiated F9 cells that resemble tight junction-bearing viceral endoderm resulting from stimulation with retinoc acid and mediated by RXRalpha and RARgamma. Claudin-6 is absent in mouse brain and lung. The human claudin-6 gene maps to chromosome 16p13.3.

Alternate Names

Claudin6, CLDN6, Skullin 2

Gene Symbol

CLDN6

Additional Claudin-6 Products

Product Documents for Claudin-6 Antibody (9H8) - BSA Free

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Certificate of Analysis

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Product Specific Notices for Claudin-6 Antibody (9H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.