CLIC4 Antibody - BSA Free

Immunohistochemistry-Paraffin: CLIC4 Antibody - BSA Free [NBP1-85574]

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Western Blot: CLIC4 Antibody - BSA Free [NBP1-85574]

Analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Immunohistochemistry-Paraffin: CLIC4 Antibody - BSA Free [NBP1-85574]

Immunohistochemistry analysis in human kidney and skeletal muscle tissues using HPA008019 antibody. Corresponding CLIC4 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: CLIC4 Antibody - BSA Free [NBP1-85574]

Staining of human kidney, liver, pancreas and skeletal muscle using Anti-CLIC4 antibody NBP1-85574 (A) shows similar protein distribution across tissues to independent antibody NBP2-49321 (B).

Western Blot: CLIC4 Antibody - BSA Free [NBP1-85574] -

The N-terminal putative transmembrane domain (PTMD) is required for CLIC4 re-localization and Rac1 activation in response to S1P. (A) cartoon representation of the HA-tagged (∆PTMD)CLIC4 construct, and western blot analysis and quantification of its expression in HUVEC (see Methods), as compared to full-length HA-CLIC4. (B) Immunofluorescence of full-length HA-tagged CLIC4 (top) and (∆PTMD)CLIC4, and their localization after treatment with S1P. Yellow arrows denote PM localization. Scale bar represents 50 um in all panels. (C) the (∆PTMD)CLIC4 construct does not rescues the Rac1 activation defect caused by CLIC4KD, as assessed by G-LISA. (D) the (∆PTMD)CLIC4 construct does not rescue the TEER defect caused by CLIC4KD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40235733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.