CLIC4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92908

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CLIC4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human CLIC4 (NP_039234.1). MALSMPLNGLKEEDKEPLIELFVKAGSDGESIGNCPFSQRLFMILWLKGVVFSVTTVDLKRKPADLQNLAPGTHPPFITFNSEVKTDVNKIEEFLEEVLCPPKYLKLSPKHPESNTAGMDIFAKFSAYIKNSRPEANEALERGLLKTLQKLDEYLNSPLPDEIDENSMEDIKFSTRKFLDGNEMTLADCNLLPKLHIVKVVAKKYRNFDIPKEMTGIWRYLTNAYSRDEFTNTCPSDKEVEIAYSDVAKRLTK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CLIC4 Antibody - BSA Free

Western Blot: CLIC4 AntibodyBSA Free [NBP2-92908]

Western Blot: CLIC4 AntibodyBSA Free [NBP2-92908]

Western Blot: CLIC4 Antibody [NBP2-92908] - Analysis of extracts of various cell lines, using CLIC4 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: CLIC4 Antibody - BSA Free [NBP2-92908]

Immunocytochemistry/ Immunofluorescence: CLIC4 Antibody - BSA Free [NBP2-92908]

Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP2-92908] - Analysis of A-549 cells using CLIC4. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: CLIC4 Antibody - BSA Free [NBP2-92908]

Immunohistochemistry-Paraffin: CLIC4 Antibody - BSA Free [NBP2-92908]

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-92908] - Rat kidney using CLIC4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: CLIC4 Antibody - BSA Free [NBP2-92908]

Immunohistochemistry-Paraffin: CLIC4 Antibody - BSA Free [NBP2-92908]

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-92908] - Human esophageal cancer using CLIC4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: CLIC4 Antibody - BSA Free [NBP2-92908]

Immunohistochemistry-Paraffin: CLIC4 Antibody - BSA Free [NBP2-92908]

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-92908] - Mouse kidney using CLIC4 Rabbit pAb dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for CLIC4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CLIC4

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq]

Alternate Names

chloride intracellular channel 4, chloride intracellular channel 4 like, chloride intracellular channel protein 4, CLIC4L, DKFZp566G223, FLJ38640, H1, huH1, Intracellular chloride ion channel protein p64H1, MTCLIC, P64H1

Gene Symbol

CLIC4

Additional CLIC4 Products

Product Documents for CLIC4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CLIC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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