CLIP170 Antibody (CL4851) - BSA Free
Novus Biologicals | Catalog # NBP2-61144
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL4851
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P30622, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CLIP170 Antibody (CL4851) - BSA Free
![Western Blot: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.rndsystems.com/images/products/CLIP170-Antibody-CL4851-Western-Blot-NBP2-61144-img0010.jpg "Western Blot: CLIP170 Antibody (CL4851) [NBP2-61144]")
Western Blot: CLIP170 Antibody (CL4851) [NBP2-61144]
Western Blot: CLIP170 Antibody (CL4851) [NBP2-61144] - Analysis in human cell line HDLM-2 and human cell line U-2197.![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.rndsystems.com/images/products/CLIP170-Antibody-CL4851-Immunohistochemistry-Paraffin-NBP2-61144-img0004.jpg "Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]")
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of human kidney shows moderate to strong cytoplasmic immunoreactivity in renal tubules.![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.rndsystems.com/images/products/CLIP170-Antibody-CL4851-Immunohistochemistry-Paraffin-NBP2-61144-img0001.jpg "Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]")
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of human cerebellum shows moderate cytoplasmic immunoreactivity in a subset of neuronal cells.![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.rndsystems.com/images/products/CLIP170-Antibody-CL4851-Immunohistochemistry-Paraffin-NBP2-61144-img0002.jpg "Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]")
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.rndsystems.com/images/products/CLIP170-Antibody-CL4851-Immunohistochemistry-Paraffin-NBP2-61144-img0003.jpg "Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]")
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of human placenta shows strong cytoplasmic immunoreactivity in trophoblast.![CLIP170 Antibody (CL4851) - BSA Free Immunocytochemistry/ Immunofluorescence: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.rndsystems.com/images/products/nbp2-61144_-immunocytochemistry-immunofluorescence-639174077036597085.jpg "Immunocytochemistry/ Immunofluorescence: CLIP170 Antibody (CL4851) [NBP2-61144]")
Immunocytochemistry/ Immunofluorescence: CLIP170 Antibody (CL4851) [NBP2-61144]
Staining of A549 cells, showing specific staining in microtubule ends in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).Applications for CLIP170 Antibody (CL4851) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:10000 - 1:20000
Immunohistochemistry-Paraffin
1:10000 - 1:20000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CLIP170
Alternate Names
CAP-GLY domain containing linker protein 1, CLIP, CLIP170, CLIP-170Reed-Sternberg intermediate filament-associated protein, CYLN1MGC131604, cytoplasmic linker 1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, Reed-Steinberg cell-expressed intermediate filament-associated protein, Restin, restin (Reed-Steinberg cell-expressed intermediate filament-associated protein), RSNCAP-Gly domain-containing linker protein 1
Gene Symbol
CLIP1
Additional CLIP170 Products
Product Documents for CLIP170 Antibody (CL4851) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CLIP170 Antibody (CL4851) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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