Clostridium Difficile Toxin A Antibody - BSA Free

Novus Biologicals | Catalog # NB100-62473

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Toxin A/TcdA Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the amino acids 2123 - 2132 of the native molecule conjugated to Keyhole limpet Haemocyanin. Peptide sequence: CTIDGKKYYFN

Epitope

CTIDGKKYYFN

Localization

Secreted

Specificity

NB100-62473 is specific for Clostridium difficile Toxin A, a gram-positive motile bacterium which is the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density, and the administration of antibiotics. Toxin A (TcdA) is a large exotoxin which is an essential virulence factor for C. difficile infection. TcdA has a molecular weight of 308kD and is a glucosyltransferase, which irreversibly inactivates the Ras family of small GTPases to disrupt many important signalling pathways.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Clostridium Difficile Toxin A Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000
Application Notes
Use in Western blot reported in scientific literature (PMID: 27246573).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

5.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Toxin A/TcdA

Clostridia are toxin-producing anaerobic bacteria that cause a number of serious diseases, including tetanus, botulism, and tissue infections. Clostridia normally inhabit the human intestinal tract, soil, and decaying vegetation. All species of clostridia produce toxins. Some clostridial diseases, such as botulism and the various diarrheal illnesses, result solely from the toxin without any bacterial invasion of tissue. In other clostridial diseases, such as tetanus and clostridial wound infections, there is both tissue invasion and toxin production.

Alternate Names

ToxA, Toxin A

UniProt

P16154 (C. difficile)

Additional Toxin A/TcdA Products

Product Documents for Clostridium Difficile Toxin A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Clostridium Difficile Toxin A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Citations for Clostridium Difficile Toxin A Antibody - BSA Free

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FAQs for Clostridium Difficile Toxin A Antibody - BSA Free

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  • Q: I am seeking monoclonal antibodies specific for the C Terminal binding regions of clostridum difficile toxins A and B (aa1852-2366)?

    A: I would recommend catalog # NB100-62473. Although it is a polyclonal, it is the only one that we know binds to the C-Terminal portion of the protein. The other antibodies we have against both targets are synthesized from the full length sequence. We do not routinely epitope map our antibodies so we cannot be certain where they are binding. This polyclonal is synthesized against a small 10 amino acid region of the protein so it should be highly specific to that location.

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