Rat Clusterin/APOJ ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-75388

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat CLU in samples. No significant cross-reactivity or interference between Rat CLU and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.77%

Inter-Assay Precision (Precision between assays) CV% 5.28%

Recovery for Rat Clusterin/APOJ ELISA Kit (Colorimetric)

Recovery

86-108%

Linearity

Rat Clusterin/APOJ ELISA Kit (Colorimetric)

Scientific Data Images for Rat Clusterin/APOJ ELISA Kit (Colorimetric)

Rat Clusterin/APOJ ELISA Kit (Colorimetric)

ELISA: Rat Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-75388] -

ELISA: Rat Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-75388] - Standard Curve Reference

Kit Contents for Rat Clusterin/APOJ ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Clusterin

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP-40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid poly-peptide that is posttranslationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, alpha and beta, are associated through disulfide bonds. The beta subunit (also called ApoJalpha) corresponds to residues 23-227. The alpha subunit (also called ApoJbeta) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences beta-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer's disease pathogenesis.

Alternate Names

APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2

Gene Symbol

CLU

Additional Clusterin Products

Product Documents for Rat Clusterin/APOJ ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat Clusterin/APOJ ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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