CMP kinase Overexpression Lysate
Novus Biologicals | Catalog # NBL1-09298
![Western Blot: CMP kinase Overexpression Lysate [NBL1-09298]](https://resources.rndsystems.com/images/products/CMP-kinase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09298-img0002.jpg "Western Blot: CMP kinase Overexpression Lysate [NBL1-09298]")
Key Product Details
Species
Applications
Product Summary for CMP kinase Overexpression Lysate
CMP kinase Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC204856
Accession#: NM_016308
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).
Product Specifications
Application Notes
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Scientific Data Images for CMP kinase Overexpression Lysate
Western Blot: CMP kinase Overexpression Lysate [NBL1-09298]
Western Blot: CMP kinase Overexpression Lysate (Adult Normal) [NBL1-09298] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CMP kinase.Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: CMP kinase
Alternate Names
Gene Symbol
Additional CMP kinase Products
Product Documents for CMP kinase Overexpression Lysate
Certificate of Analysis
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Product Specific Notices for CMP kinase Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars